Journal: Journal of Biological Chemistry
Article Title: Impaired Activation of Phosphatidylinositol 3-Kinase by Leptin Is a Novel Mechanism of Hepatic Leptin Resistance in Diet-induced Obesity
doi: 10.1074/jbc.m401546200
Figure Lengend Snippet: FIG. 7. Effects of diet-induced obesity on hepatic leptin recep- tor expression. Livers were isolated from 18 h of fasted lean or diet-induced obese rats. Leptin receptor protein and leptin receptor mRNA were measured by Western blot and RPA, respectively, as de- scribed under “Experimental Procedures.” The figure shows represent- ative autoradiographs of ObRa protein and ObRtot mRNA in liver from lean (LEAN) and diet-induced obese (OBESE) rats. n a minimum of 6 livers/group.
Article Snippet: Leptin receptor protein level was determined by immunoblotting using an -leptin receptor antibody (K-20, 1:3000, Santa Cruz Biotechnology, Santa Cruz, CA) and -goat IgG-HRP (1:5000, Santa Cruz Biotechnology).
Techniques: Expressing, Isolation, Western Blot